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Utero‐relaxant effect of PDE4‐selective inhibitor alone and in simultaneous administration with β2‐mimetic on oxytocin‐induced contractions in pregnant myometrium
Author(s) -
Franova Sona,
Janicek Frantisek,
Visnovsky Jozef,
Dokus Karol,
Zubor Pavol,
Sutovska Martina,
Nosalova Gabriela
Publication year - 2009
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2008.00839.x
Subject(s) - rolipram , salbutamol , oxytocin , myometrium , medicine , uterine contraction , agonist , endocrinology , tocolytic agent , muscle relaxant , phosphodiesterase , in utero , pharmacology , uterus , preterm labor , chemistry , receptor , fetus , pregnancy , enzyme , biology , biochemistry , asthma , genetics
Background: The objective of the study was to observe the effect of rolipram, the prototype phosphodiesterase 4 selective inhibitor, on oxytocin‐induced contractions of human term myometrial strips, and compare the effect with salbutamol, β 2 ‐adrenergic agonist, in single and the simultaneous application. Methods: Human myometrium was obtained from pregnant women in term that had a term delivery by the caesarian section. Myometrial strips were excised from the lower uterine segment and placed into an organ‐bath with Krebs‐Henseleit buffer. The mean peak amplitude of contraction (mN) of the myometrial smooth muscle to the doses of oxytocin (10 −6 mmol/L −1 ) with subsequent single administration of rolipram (10 −4 mmol/L −1 ), salbutamol (10 −4 mmol/L 1 ) and simultaneous administration of rolipram and salbutamol (both 10 −4 mmol/L −1 ), was used as a parameter of myometrial reactivity. Results: Rolipram alone decreased the oxytocin‐induced contractile amplitude to 47.98%, single salbutamol application resulted in amplitudinal decrease to 56.07%, and the combination of both compounds in their simultaneous administration resulted in the decrease of oxytocin‐induced contractile amplitude to 29.1%. Conclusion: Our data are consistent with previous studies of the enhanced efficiency of the β 2 ‐adrenergic agonist, when administered together with the phosphodiesterase 4‐inhibitor. Moreover we have shown that rolipram alone has a more profound effect on oxytocin‐induced contractions than salbutamol alone.