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Total peroxyl radical‐trapping ability and anti‐oxidant vitamins of the umbilical venous plasma and the placenta in pre‐eclampsia
Author(s) -
Kim Yoon Ha,
Kim Cheol Hong,
Cho Moon Kyoung,
Kim Ki Min,
Lee So Yoon,
Ahn Bong Whan,
Yang Sung Yeul,
Kim Seok Mo,
Song Tae Bog
Publication year - 2006
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2006.00348.x
Subject(s) - placenta , medicine , lipid peroxide , vitamin e , endocrinology , eclampsia , venous blood , tocopherol , pregnancy , thiobarbituric acid , preeclampsia , ascorbic acid , andrology , fetus , chemistry , oxidative stress , lipid peroxidation , antioxidant , biochemistry , biology , food science , genetics
Aim: Our purpose was to investigate lipid peroxide levels, total peroxyl radical‐trapping anti‐oxidative parameter (TRAP) values, and anti‐oxidant vitamin levels in umbilical venous plasma and placental tissues, and to evaluate their roles in the pathophysiology of pre‐eclampsia. Methods: Samples of umbilical venous plasma and placental tissue homogenates were obtained from 23 normal and 18 pre‐eclamptic women at between 33 and 40 weeks’ gestation. The enzyme‐linked immunosorbent assay method was used to assay α‐tumor necrosis factor (TNF‐α), and lipid peroxide levels were measured by thiobarbituric acid reaction. The TRAP values were measured using the modified Wayner's method. Ascorbic acid, retinol α‐tocopherol and γ‐tocopherol were measured by high performance liquid chromatography. Results: Levels of TNF‐α in placental tissue homogenates of women with pre‐eclampsia were significantly higher than those of women with normal pregnancy (21.4 ± 3.39 v. 10.3 ± 1.06 pg/mL, P < 0.05). Lipid peroxide levels in umbilical venous plasma and placental tissue homogenates of women with pre‐eclampsia were significantly higher than those of women with normal pregnancy (10.3 ± 1.1 v. 5.85 ± 0.53, P < 0.01, 5.14 ± 0.40 v. 3.99 ± 0.33 nmol/mg protein, P < 0.05, respectively). The TRAP values in umbilical venous plasma and placental tissue homogenates of women with pre‐eclampsia were significantly lower than those of women with normal pregnancy (0.39 ± 0.02 v. 0.45 ± 0.02, P < 0.05, 0.27 ± 0.02 v. 0.34 ± 0.03 mM, P < 0.05, respectively). Ascorbic acid levels in umbilical venous plasma and placental tissue homogenates of women with pre‐eclampsia were significantly lower than those of women with normal pregnancy (325.4 ± 50.4 v. 543 ± 73.8, P < 0.05, 219.0 ± 21.0 v. 333.3 ± 32.6 nmol/mL, P < 0.05, respectively). Conclusions: The above results suggest that increased oxidative stress in the placenta is involved in the pathophysiology of pre‐eclampsia, and ascorbic acid may act as an important preventative factor in the development of pre‐eclampsia.