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Influence of α‐tumor necrosis factor and β‐interleukin‐1 on production of angiogenetic factors and thymidine phosphorylase activity in immortalized human decidual fibroblasts in vitro
Author(s) -
Hayashi Takashi,
Matsuoka Kikumi,
Saitoh Masahiro,
Takeda Satoru,
Kimura Masayuki
Publication year - 2006
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2006.00347.x
Subject(s) - decidua , angiogenesis , thymidine phosphorylase , tumor necrosis factor alpha , vascular endothelial growth factor , basic fibroblast growth factor , decidual cells , cytokine , endocrinology , medicine , cell culture , growth factor , cancer research , biology , fetus , pregnancy , vegf receptors , receptor , placenta , genetics , cancer
Aim: The aim of the present study was to investigate regulatory mechanisms of angiogenesis in the decidua using immortalized human decidual fibroblasts. Methods: A sample of decidual fibroblasts was taken from a woman in early pregnancy. A cell line, DE‐1, was established by infecting the decidual fibroblasts with the simian virus 40 large T antigen. Using this cell line, the ability to produce vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), β‐transforming growth factor (TGF‐β), and thymidine phosphorylase (TP) activity was investigated using immunohistochemistry, and the influences of β‐interleukin‐1 (IL‐1β) and α‐tumor necrosis factor (TNF‐α) on these angiogenetic factors was investigated using enzyme‐linked immunosorbent assays. Furthermore, the effects of TNF‐α on proliferative capacity and apoptosis induction in DE‐1 were studied. Results: It was demonstrated that DE‐1 produced all of these angiogenetic factors. The production of VEGF, bFGF and TGF‐β respectively was enhanced by both IL‐1β and TNF‐α. TP activity was increased by TNF‐α, but no increase was observed as a result of IL‐1β. It was shown that TNF‐α suppressed the proliferation of DE‐1 cells and significantly increased the percentage of apoptotic cells. Conclusion: It is suggested that IL‐1β and TNF‐α stimulate decidual fibroblasts to up‐regulate angiogenesis in the human decidua.