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The Application of Magnetic Cell Sorter (MACS) to Detect Fetal Cells in Maternal Peripheral Blood
Author(s) -
Fukushima Akimune,
Utsugisawa Yukari,
Wada Yuko,
Mizusawa Noriko,
Horiuchi Saburo,
Kagabu Teruo
Publication year - 2001
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2001.tb01240.x
Subject(s) - medicine , gestation , fetus , obstetrics , prenatal diagnosis , peripheral blood , pregnancy , gynecology , first trimester , predictive value , nucleated red blood cell , andrology , biology , genetics
Objectives: The aim of the present study was to investigate the effectiveness of sorting fetal nucleated red blood cells (FNRBC) from maternal peripheral blood, particularly during early gestation periods, by a combination of specific gravity centrifugation and magnetic cell sorter (MACS). Methods: Without prior knowledge of the gender of the fetus, we determined gender by analyzing a Y‐chromosome specific sequence by nested‐PCR, using 10 ml of the peripheral blood of healthy primigravida women at different stages of gestation (first trimester: n = 17, second trimester: n = 13, and third trimester: n = 19). The results of this prenatal sex determination were compared to the sex of newborns. Results: The specificity, sensitivity, positive predictive value (PPV) and negative predictive value (NPV) of the present method during the first trimester were 100, 81.8, 100, and 75%, respectively; during the second trimester, 80, 50, 80, and 50%, respectively; and during the third trimester, 25, 63.6, 53.8, and 33.3%, respectively. Conclusion: The results show that this prenatal sex determination method has a highly accurate diagnostic rate during the first trimester, suggesting that it could be developed as a practical, non‐invasive prenatal diagnostic technique for use during early gestation periods.