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The Presence and Role of the Dopamine DA‐2 Receptor in the Human Decidua
Author(s) -
Arai Fujimi,
Kishimoto Yasuo,
Tada Katsuhiko,
Kondo Yuji,
Kudo Takafumi
Publication year - 2000
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2000.tb01356.x
Subject(s) - decidua , sulpiride , dopamine receptor , receptor , dopamine , endocrinology , spiperone , medicine , dopaminergic , andrology , biology , fetus , placenta , pregnancy , genetics
Objectives: Our objectives were to identify the presence of the dopamine DA‐2 receptor in human decidua and to study its function in human parturition. Methods: Human term decidual tissues were obtained during vaginal delivery and then homogenized. The P3 fraction was prepared for a radiolabeled receptor assay with [ 3 H] spiperone as the ligand. Human decidual tissues obtained at cesarean section before the onset of labor were incubated in Krebs‐Ringer buffer at 37°C for 30 minutes in the presence of dopamine with or without (–)‐sulpiride. The level of prostaglandin (PG) F in the medium was measured with a RIA kit. Differences were assessed with the Wilcoxon non‐parametric test. Results: Scatchard analysis showed a single class of binding sites having an equilibrium dissociation constant (K d ) of 2.25 + 0.59 n m (mean + SD) and a maximum binding capacity (B max ) value of 166.5 + 77.7 fmol/mg protein ( n = 3). Dopamine significantly increased the production of PGF. This stimulatory effect of dopamine was suppressed by (–)‐sulpiride (p < 0.05; n = 7). Conclusion: The DA‐2 receptor was demonstrated in the human decidua. Dopamine can stimulate PGF production via this receptor.