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Effects of Low Oxygen Condition on the Generation of Reactive Oxygen Species and the Development in Mouse Embryos Cultured in vitro *
Author(s) -
Kwon HyuckChan,
Yang HyunWon,
Hwang KyungJoo,
Yoo JungHyun,
Kim MyungSin,
Lee ChiHyeong,
Ryu Hee Sug,
Oh Kie Suk
Publication year - 1999
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.1999.tb01177.x
Subject(s) - embryo , andrology , embryogenesis , blastomere , reactive oxygen species , oxygen , in vitro , fragmentation (computing) , microbiology and biotechnology , biology , chemistry , medicine , genetics , ecology , organic chemistry
Objective(s): To elucidate the relationship between intracellular H 2 O 2 production and embryo development in different oxygen culture conditions. Methods: Pronuclear stage embryos were obtained from C57BL/CBA F1 and ICR mice. Measurement of H 2 O 2 level was performed with 2′,7′‐dichlorodihydroflourescein diacetate and the number of blastomeres was counted after staining with 4′,6′‐diamidino‐2‐phenylindole. Results: Regardless of strains, H 2 O 2 level reached a peak at the 2‐cell stage in 20% O 2 . But in embryos cultured in 5% O 2 it was significantly lower at the 2‐cell and 4‐cell stages compared to those from 20% O 2 . The embryos cultured in 20% O 2 showed developmental delay or block, but in 5% O 2 these phenomena were overcome and the development was significantly increased with an infrequent fragmentation. Conclusions: Our data suggest that the 5% O 2 decreases the relative concentration of H 2 O 2 and results in improved embryo development in terms of quantity and quality without regard to type of strains.