Premium
Effects of All‐ trans Retinoic Acid on Choriocarcinoma Cells in vitro *
Author(s) -
Yamada Satoru,
Okamoto Tomomitsu,
Nakanishi Toru,
Nomura Seiji,
Tomoda Yutaka
Publication year - 1997
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.1997.tb00820.x
Subject(s) - choriocarcinoma , retinoic acid , methotrexate , medicine , in vitro , cell growth , cell culture , receptor , mtt assay , microbiology and biotechnology , endocrinology , biology , biochemistry , genetics
Objective : To determine the activities of all‐ trans retinoic acid (RA) on choriocarcinoma cells in vitro. Methods : The antiproliferative effect of all‐ trans RA on 4 choriocarcinoma cell lines was measured by the MTT assay. The effect of all‐ trans RA combined with methotrexate or actinomycin‐D was then examined. The effect of all‐ trans RA on hCG secretion was also studied. The gene expression of retinoic acid receptors (RARs) was examined by RT‐PCR. Results : All‐ trans RA inhibited cell proliferation dose‐ and time‐dependently; a 6‐day exposure to 1 μM all‐ trans RA suppressed the cell growth by 67.8%‐82.0% compared to the controls. An enhanced effect was observed in the combined administration of all‐ trans RA and methotrexate or actinomycin‐D. The secretion of hCG increased 4‐fold to 9‐fold by the addition of 1 μ m all‐ trans RA. RARs genes were expressed in all cell lines. Conclusion : The anticancer activity presented here appears to warrant further evaluation of all‐ trans RA as adjuvant therapy for choriocarcinoma.