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Regulation of Neutrophil Superoxide Generation by Alpha‐Tocopherol in Human Peripheral and Umbilical‐Cord Blood
Author(s) -
Ando Masaki,
Yoshioka Tamotsu,
Akiyama Jitsuo,
Kudo Takafumi
Publication year - 1996
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.1996.tb01064.x
Subject(s) - umbilical cord , superoxide , zymosan , medicine , protein kinase c , annexin , stimulation , respiratory burst , andrology , microbiology and biotechnology , immunology , endocrinology , biochemistry , phosphorylation , flow cytometry , chemistry , in vitro , enzyme , biology
Objectives : Alfa‐tocopherol (VE) inhibits protein phosphorylation and affects protein kinase C (PKC)‐dependent superoxide (O 2 ‐) generation of phagocytic cells. Because umbilical‐cord blood contains low concentrations of VE, we studied the effects of VE on stimulation‐dependent O 2 generation of neutrophils derived from the healthy human peripheral circulation (HPMNs) and the umbilical cord (UCBNs). Methods : O 2 ‐ generation and VE content were assayed by reduction of ferricytochrome c and by HPLC with an electrochemical detector, respectively. Neutrophil‐specific annexin protein was identified by an immunological method. Results : Both types of neutrophils generate significant amounts of PKC‐dependent O 2 ‐ generation, but weak PKC‐independent O 2 ‐ generation, except for opsonized zymosan‐stimulated O 2 ‐ generation in UCBNs. The VE content of UCBNs was similar to that of HPMNs although umbilical‐cord blood contains a low concentration of VE. A neutrophil‐specific annexin protein was also found in the UCBNs Conclusion : UCBNs have a greater ability to incorporate VE than HPMNs do, but have similar general characteristics of stimulation‐dependent O 2 ‐ generation to those of HPMNs.