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The Role of Superoxide Dismutase in the Human Ovary and Fallopian Tube *
Author(s) -
Tamate Kenichi,
Sengoku Kazuo,
Ishikawa Mutsuo
Publication year - 1995
Publication title -
journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1340-9654
DOI - 10.1111/j.1447-0756.1995.tb01029.x
Subject(s) - ovary , fallopian tube , theca , superoxide dismutase , follicular fluid , ovulation , follicular phase , luteal phase , medicine , endocrinology , andrology , chemistry , ovarian follicle , theca interna , biology , oocyte , anatomy , oxidative stress , microbiology and biotechnology , hormone , embryo
Objectives : To investigate the localization of manganese superoxide dismutase (Mn‐SOD) and copper‐zinc superoxide dismutase (Cu, Zn‐SOD) in the human ovary and fallopian tube, and to examine the role of superoxide radicals and SODs in the human ovulatory process. Methods : Using immunohistochemical methods, we studied the localization of SODs in 22 human ovaries, in 18 fallopian tubes, and in aspirated granulosa cells. We measured, by enzyme‐linked immunosorbent assay, the concentrations of SODs in follicular fluid taken from 94 IVF patients. Results : Mn‐SOD was found in granulosa, in theca and luteal cells, and in fallopian tubes. Cu, Zn‐SOD was localized in theca and luteal cells. The concentration of Cu, Zn‐SOD in follicular fluid in the high‐progesterone group (11.3 + 4.2 ng/m l ) was significantly less than in the low‐progesterone group (24.5 + 19.5) (p < 0.05). Conclusion : Mn‐SOD and Cu, Zn‐SOD have different localizations and actions in human ovaries and fallopian tubes. The superoxide radical‐SOD system might play an important role in ovulation and in the luteal function of the human ovary.