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Immunohistochemical Expression of Glutathione S‐Transferase π (GST‐π) and Chemotherapy Response in Malignant Ovarian Tumors
Author(s) -
Hirazono Kenichi,
Shinozuka Takao,
Kuroshima Yoshio,
Itoh Hitoshi,
Kawai Kenji
Publication year - 1995
Publication title -
journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1340-9654
DOI - 10.1111/j.1447-0756.1995.tb01015.x
Subject(s) - immunohistochemistry , immunoperoxidase , cisplatin , cyclophosphamide , glutathione s transferase , chemotherapy , medicine , cancer , antibody , polyclonal antibodies , cancer research , oncology , pathology , glutathione , biology , immunology , monoclonal antibody , enzyme , biochemistry
Objectives: To clarify the relationship between the expression of GST‐π and the therapeutic effects of high‐dose combination chemotherapy with support by autologous bone‐marrow transplantation (ABMT). Methods: Expression of GST‐π in tissue specimen were analyzed by indirect immunoperoxidase methods using anti‐GST‐π polyclonal antibody. Results: (1) Eighteen of 36 cases (50%) tested prior to administration of any chemotherapeutic agents were found to be positive in terms of immunohistochemical expression of GST‐π in the cancer cells. (2) The rate of GST‐π expression was elevated following administration of cisplatin, adriamycin, and cyclophosphamide, which suggests that administration of such anti‐cancer agents is capable of inducing expression of GST‐π in cancer cells. (3) GST‐π negative patients exhibit better survival rates than do GST‐π positive patients (Kaplan Meier method: p < 0.05‐0.001). Conclusions: Analysis of GST‐π obtained at primary surgery can serve as a valid marker in anticipating chemotherapeutic effects and long‐term prognoses.