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Immunohistochemical Detection of p53 Overexpression in Formalin‐Fixed, Paraffin‐Embedded Sections of Endometrial Carcinoma
Author(s) -
Iwai Kyouko,
Hachisuga Toru,
Iwasaka Tsuyoshi,
Sugimori Hajime
Publication year - 1995
Publication title -
journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1340-9654
DOI - 10.1111/j.1447-0756.1995.tb00918.x
Subject(s) - immunohistochemistry , frozen section procedure , pathology , polyclonal antibodies , staining , carcinoma , antigen retrieval , concordance , antibody , chemistry , medicine , biology , immunology
Objective : In the part, immunohistochemical detection of p53 has been possible only in the case of fresh‐frozen tissues. However using CM1 and an enhanced method based on microwave heating and protease digestion enabled the immunohistochemical detection of p53 overexpression in formalin‐fixed, paraffin‐embedded sections of endometrial carcinoma, although very few positive stainings were obtained without such an enhanced treatment. To evaluate the enhancement method, a comparative study was performed on frozen materials and paraffin‐enbedded samples. Methods : Fifty‐three frozen materials, including normal endometrial glands, endometrial hyperplasias and carcinomas, and paraffin‐embedded samples from the same patients were used in this study. PAb1801 (mouse monoclonal antibody) was used to analyse p53 overexpression in the frozen sections, while CM1 (rabbit polyclonal anti‐p53 antibody) was used for the paraffin sections. Results : PAb1801 and CM1 staining on normal endometriums and endometrial lesions showed a significant degree of concordance between frozen and paraffin‐embedded materials. Conclusion : Enhanced immunodetection of p53 in paraffin‐embedded tissues will provide a useful alternative to the usual fresh‐tissue assay.