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1C5 Antigen: Cancer‐Associated Protein Expressed by Cervical Adenocarcinomas and β‐Casein
Author(s) -
Horimoto Emi,
Uede Toshimitsu,
Koizumi Motoiki,
Kikuchi Kokichi,
Kudo Ryuichi
Publication year - 1994
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1994.tb00477.x
Subject(s) - antigen , cervical cancer , casein , cancer , biology , cancer research , immunology , genetics , food science
The monoclonal antibody 1C5 reacts with an antigenic determinant present in 90% of the cases of adenocarcinoma of the uterine cervix. The antigen denned by the 1C5 antibody exhibits immunological characteristics similar to those of skim milk (bovine buttermilk). lC5‐defined antigen obtained from tumor extract and skim milk binds specifically to wheat germ agglutinin (WGA) lectin. The lC5‐defined antigenic activity of a WGA lectin‐bound fraction was eluted at 0.7‐0.8 m NaCl off a Mono Q column. Use of an inhibition assay and a dot immunobinding assay revealed that the antigenic epitope defined by the 1C5 MoAb from skim milk exists within the first 28 amino acids of the β‐casein peptide.