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Origin of Macrophage Colony‐Stimulating Factor (M‐CSF) and Granulocyte Colony‐Stimulating Factor (G‐CSF) in Amniotic Fluid
Author(s) -
Saito Mami,
Saito Shigeru,
Nakagawa Tomoyoshi,
Ichijo Motohiko,
Motoyoshi Kazuo
Publication year - 1992
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1992.tb00331.x
Subject(s) - amniotic fluid , cerebrospinal fluid , granulocyte colony stimulating factor , fetus , granulocyte macrophage colony stimulating factor , colony stimulating factor , urine , macrophage colony stimulating factor , staining , medicine , andrology , endocrinology , chemistry , biology , pathology , pregnancy , macrophage , cytokine , haematopoiesis , biochemistry , in vitro , stem cell , microbiology and biotechnology , genetics , chemotherapy
A large amount of M‐CSF and G‐CSF exists in human amniotic fluid and both are considered to have some physiological affect on maintaining pregnancy. We therefore examined the source of M‐CSF and G‐CSF found in the amniotic fluid. The average level of M‐CSF in the amniotic fluid of patients without complications was 17.3±8.5 ng/ml and that of G‐CSF 1.85±1.72 ng/ml, both being high values. In neonatal urine, the average level of M‐CSF was also very high, 144.3 ±97.0 ng/ml, but that of G‐CSF was below the determination limit of 60 pg/ml. Immunohistochemical staining indicated that production of M‐CSF and G‐CSF was localized in the epithelial cells of fetal membrane. On the basis of the above observations, M‐CSF was found to derive from neonatal urine and the epithelial cells of fetal membrane, and G‐CSF from the epithelial cells of fetal membrane.