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EGF Rapidly Stimulates Tyrosine Phosphorylation in Cultured Endometrial Cells
Author(s) -
Ishihara Satoko,
Taketani Yuji,
Mizuno Masahiko
Publication year - 1991
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1991.tb00287.x
Subject(s) - tyrosine phosphorylation , phosphorylation , paracrine signalling , autocrine signalling , signal transduction , epidermal growth factor , microbiology and biotechnology , tyrosine , biology , endocrinology , medicine , chemistry , biochemistry , receptor
Recent increasing evidence suggests that EGF has a role in modulating the differentiated functions of human endometrial cells in an autocrine/paracrine fashion. To explore the signal transduction pathway of EGF in endometrial cells, we used cultured human endometrial cells to examine whether EGF induces tyrosine‐phosphorylation. EGF phosphorylated the 175 kDa protein on tyrosine residues within 10 seconds of stimulation. EGF induced tyrosine phosphorylation at as low as 0.1 ng/m l with the maximal effect occurring at 10 ng/m l. Estradiol was shown to enhance the phosphorylation by EGF in this system. These results thus suggest that tyrosinephosphorylation might be an important step in the signal transduction of EGF in human endometrial cells. Furthermore, the observed stimulatory action of estradiol on tyrosine‐phosphorylation by EGF might provide a clue in the elucidation of the cellular mechanism of estrogen action in endometrium.