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An ADPase‐Like Substance in Placental Extracts
Author(s) -
Matsumoto Takafumi,
Sugiyama Youichi,
Deguchi Katsumi,
Okano Kanemichi
Publication year - 1990
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1990.tb00237.x
Subject(s) - aminopeptidase , platelet , chemistry , adenosine , size exclusion chromatography , biochemistry , placenta , leucine , sepharose , affinity chromatography , column chromatography , gel permeation chromatography , adenosine diphosphate , enzyme , chromatography , platelet aggregation , biology , fetus , medicine , amino acid , pregnancy , genetics , organic chemistry , polymer
The inhibitory effect of human placenta on ADP‐induced platelet aggregation was examined. Placental extracts were purified by chromatography and gel filtration. The eluted fractions were subjected to affinity chromatography with Bestatin AH‐Sepharose to obtain a more purified substance. A substance markedly inhibited platelet aggregation induced by ADP, and also exhibited high placental leucine aminopeptidase (P‐LAP) activity. Preincubation of sample with ADP abolished the platelet aggregatory effect. Thin‐layer chromatography (TLC) analysis revealed that the substance hydrolyzed ADP to AMP. The potent anti‐platelet aggregating activity of placental extracts appears to be due to ADPase‐like action. This action may be one of the controlling factors of hemostasis in fetoplacental circulation. Key words: human placenta, platelet aggregation, leucine aminopeptidase, adenosine diphosphatase (ADPase)