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Establishment and Characterization of CA 125 Producing Cell Line (OMC‐2) Originating from a Human Endometrial Adenocarcinoma
Author(s) -
Yamada Takashi,
Ueda Masatsugu,
Maeda Takayoshi,
Okamoto Yoshiaki,
Miyawaki Yoshitaka,
Otsuki Yoshinori,
Ueki Minoru,
Sugimoto Osamu
Publication year - 1989
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1989.tb00206.x
Subject(s) - cell culture , adenocarcinoma , contact inhibition , aneuploidy , carcinoma , cell , endometrium , endometrial cancer , ploidy , cancer research , biology , pathology , medicine , endocrinology , cancer , chromosome , genetics , gene
Abstract A new human endometrial carcinoma cell line, designated OMC‐2, was established from the endometrial adenocarcinoma of a 59‐year‐old woman. This cell line has grown well for 51 months and has been subcultured more than 50 times. Monolayer cultured cells are polygonal in shape, showing a pavement‐like arrangement and a piling up tendency without contact inhibition. The chromosomal number shows aneuploidy and the modal chromosomal number is in the diploid range. The cells were transplanted into the subcutis of nude mice and produced tumors resembling the original tumor. 1X10 5 OMC‐2 cells produced CA 125 (184–682 U) during 19 days in culture media. CA 125 was demonstrated immunohistochemically in the original tumor, heterotransplanted tumor, and OMC‐2 cells. The cells contain no estrogen or progesterone receptors. Twenty‐nine other reports of endometrial carcinoma cell lines are reviewed.