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The Effect of an Anti‐Progestin Compound (RU486) on Gonadotropin Release from Rat Anterior Pituitary Cells in vitro
Author(s) -
Arakawa Satoko,
Ohkawa Tomi,
Kambegaaw Akira,
Okinaga Shoichi,
Arai Kiyoshi
Publication year - 1988
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1988.tb00101.x
Subject(s) - norethisterone , medicine , endocrinology , anterior pituitary , luteinizing hormone , progestin , radioimmunoassay , chemistry , in vitro , gonadotropin , hormone , health services , population , biochemistry , environmental health
Anterior pituitary cells from castrated female rats were cultured in a medium with or without progesterone (P 4 ), estradiol‐17 β (E 2 ), 17 β‐hydroxy‐11 β‐(4‐dimethylaminophenyl)‐17‐(prop‐1‐ynyl)‐estra‐4,9‐dien‐3‐one (RU486) or norethisterone (NE). Three days later, the media were changed and the cells were incubated further with or without luteinizing hormone releasing hormone (LHRH) (10 ‐6 ‐10 ‐12 M) for 3 hours. LH and FSH released in the media were measured by radioimmunoassay (RIA). At a high concentration of LHRH (>10 ‐8 M), E 2 (10 ‐9 M) increased the release of LH and FSH significantly. When P 4 (10 ‐6 M) was combined with E 2 for 3 days, the release of LH and FSH by E 2 was inhibited. RU486 had no direct effect on LH and FSH release. However, the effect of P 4 against E 2 , as mentioned above, was nullified by 10 ‐6 M of RU486. In the same experimental model, RU486 also inhibited the effect of norethisterone. By the use of the anti‐progestin compound, RU486, it was confirmed that progestins directly inhibited the action of E 2 at the anterior pituitary level.