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Stimulatory Action of Progesterone on the Synthesis of Glycogen in Primary Cell Culture of Human Endometrium
Author(s) -
Ishihara Satoko,
Taketani Yuji,
Mizuno Masahiko
Publication year - 1988
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1988.tb00081.x
Subject(s) - glycogen , glycogen synthase , glycogen phosphorylase , medicine , endocrinology , mifepristone , endometrium , stimulation , progesterone receptor , biology , chemistry , pregnancy , genetics , cancer , estrogen receptor , breast cancer
The effects of progesterone and its antagonist, RU486, on the synthesis of glycogen were investigated using a primary cell culture of human endometrium. Progesterone stimulated the glycogen synthesis in a dose‐dependent manner. The minimal effective dose of progesterone is 10 ‐9 M with maximal effect occurring at 10 ‐7 M. Stimulation of glycogen synthesis was apparent as early as after 6 hours of incubation. Progesterone induced the activity of glycogen synthetase and reduced the activity of glycogen phosphorylase. The addition of RU486 with progesterone completely eliminated the action of progesterone in both the synthesis of glycogen and the activity of glycogen synthetase. These results clearly demonstrate the stimulatory action of progesterone in glycogen synthesis in cultured human endometrial cells. Moreover, the antiprogesterone, RU486, is shown to counteract the progesterone action in glycogen synthesis in vitro.