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Effect of Actinomycin D and Puromycin upon Basal and LH‐Induced Steroidogenesis by Hamster Follicles in Culture
Author(s) -
Matson Phillip L.,
Collins W. P.
Publication year - 1987
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1987.tb00275.x
Subject(s) - puromycin , hamster , medicine , messenger rna , endocrinology , dactinomycin , basal (medicine) , protein biosynthesis , in vitro , biology , hormone , corpus luteum , chemistry , microbiology and biotechnology , gene , biochemistry , cycloheximide , insulin
The role of protein synthesis in modulating steroid production by preovulatory hamster follicles (591–740 μm) was investigated in vitro using selective inhibitors of transcription (actinomycin D; 8 μg/ml) and translation (puromycin; 80 μg/ml). The results showed:(i) basal oestradiol production was increased in the presence of either actinomycin D or puromycin, suggesting the existence of a protein inhibitor of oestrogen synthesis; (ii) the initial stimulatory action of LH upon steroidogenesis is abolished by puromycin but not actinomycin D, suggesting that the synthesis of new protein is required but not new mRNA; and (iii) the preovulatory ovarian follicle grown in a defined culture medium is a good model system to study the hormonal regulation of gene expression.