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Properties of Ectopic Proteins Synthesis in vitro by Human Cervical Carcinoma Cells
Author(s) -
Chao KuanChong,
Ng HeungTat,
Chang ChiChing,
Hu ChengPo,
Chang ChungMing
Publication year - 1987
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1987.tb00254.x
Subject(s) - carcinoembryonic antigen , ferritin , cell culture , in vitro , radioimmunoassay , ectopic expression , hela , cancer research , cervical cancer , cervical carcinoma , cell , cancer , chemistry , biology , microbiology and biotechnology , medicine , pathology , biochemistry , genetics
Two ectopic proteins, carcinoembryonic antigen (CEA) and ferritin, were synthesized in the culture media from a newly established cervical cancer cell line as measured by radioimmunoassay. The cervical cancer cell line, designated CC‐7T, produced CEA and ferritin ectopically in vitro in increasing quantities as cultures proceeded. In control studies, cultured fibroblasts did not have this secreting phenomenon. The amount of CEA and ferritin production by CC‐7T cells depended upon the cell density, nutrient availability, and the accumulation of waste products. Our findings indicate that CEA and ferritin were directly secreted by cervical cancer cells from cell line CC‐7T. This cell line should be a useful tool for studies of the mechanism of re‐expression of the oncodevelopmental genes which are responsible for the proteins' production. We also presume that there is a parallelism between the ectopic protein production and the advancement of the tumor process in positive cervical cancer patients making them excellent tumor markers for clinical monitoring of patients.