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Cytological Properties of Human Ovarian Carcinoma Cell Lines
Author(s) -
Ishiwata Isamu,
Ishiwata Chieko,
Nozawa Shiro,
Ishikawa Hiroshi
Publication year - 1987
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1987.tb00014.x
Subject(s) - cell culture , granulocytosis , serous cystadenocarcinoma , ovarian carcinoma , carcinoma , in vitro , nude mouse , adenocarcinoma , pathology , cancer research , chemistry , biology , medicine , granulocyte , ovarian cancer , immunology , cancer , biochemistry , genetics
Ovarian carcinomas from 30 cases were cultured, and 8 cell lines, including 4 lines of serous cystadenocarcinoma (SCAC), 2 lines of endometrioid adenocarcinoma (EAC), one line of anaplastic carcinoma (AC), and one line of clear cell carcinoma (CCC), were established. Examination of the cytobiological properties (morphology, proliferation, chromosomes, heterotransplantability, etc.) of the cell lines revealed that the cell lines maintain the characteristics of the original tumor in vitro for a long period. HTOA and HUOA, both SCAC lines of a well differentiated type, produce a large amount of CA125 and formed SCACs that were extremely similar to the original tumor in nude mice. HTBOA (AC line) and HUOCA‐II (CCC line, poorly differentiated type) were both easy to transplant to nude mice, and the tumor formed was rich in blood vessels, proliferated rapidly, and showed histology that was similar to that of the original tumor. The HTBOA cells produced granulocyte colony‐stimulating‐factor (G‐CSF) and caused granulocytosis in the mouse.