Premium
Trehalase Isozymes in Amniotic Fluid
Author(s) -
Honda Kenichi,
Ogita Sachio,
Imanaka Motoharu,
Oka Tomoko,
Matsumoto Masahiko,
Sugawa Tadashi
Publication year - 1986
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1986.tb00235.x
Subject(s) - isozyme , trehalase , amniotic fluid , fetus , meconium , gestation , biology , biochemistry , chemistry , enzyme , pregnancy , genetics
A technique has been developed for the separation of trehalase isozymes by poly‐acrylamide slab gel electrophoresis in horizontal plates. Amniotic fluid was found to contain two isozymes at middle gestation and two or three isozymes at late gestation. Meconium at middle gestation and newborn urine contain only low‐mobility isozyme. High‐mobility and medium‐mobility isozymes are thought to be formed by recombination of enzyme subunits originating from fetal meconium and urine. We also demonstrated developmental changes of trehalase isozyme in chorionic tissue. Trehalase isozymes appear to be an useful metabolic marker of fetal maturity.