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Secretion of the β‐subunit of Luteinizing Hormone in Dispersed Rat Anterior Pituitary Cells
Author(s) -
Seki Takashi,
Makino Tsunehisa,
Tanimoto Satoshi,
Izumi Shunichiro,
Iizuka Rihachi
Publication year - 1986
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1986.tb00169.x
Subject(s) - luteinizing hormone , anterior pituitary , medicine , endocrinology , gonadotropic cell , hormone , secretion , chemistry , in vitro , desensitization (medicine) , priming (agriculture) , pituitary gland , biology , receptor , biochemistry , botany , germination
Enzymatically dispersed rat anterior pituitary cells were perifused in order to investigate the secretion pattern of luteinizing hormone (LH) and its β‐subunit (LH‐β) by luteinizing hormone‐releasing hormone (LH‐RH) in vitro . Intermittent stimulations with LH‐RH induced marked release of LH and LH‐β. The self‐priming effect on either LH or LH‐β release by LH‐RH was not significant; in contrast, a desensitization effect produced by this decapeptide hormone was clearly observed in the release of these two peptide hormones. The perifusion technique was revealed to be useful in analyzing the temporal secretion of the gonadotro‐pins from the anterior pituitary cells, and the data suggest that LH‐β is released concomitantly with native LH by LH‐RH in vitro .