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Correlation Between Amniotic Fluid Lecithin‐Sphingomyelin (L/S) Ratio and Optical Density Measured after Centrifugation at Varying Forces
Author(s) -
Shimokawa Hiroshi,
Hisanaga Sachio,
Maesato Soei,
Kashiwabara Yoshiro,
Maeda Hirotaka,
Oki Satoshi,
Nakano Hitoo
Publication year - 1985
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1985.tb00744.x
Subject(s) - amniotic fluid , lecithin , sphingomyelin , optical density , wavelength , chemistry , chromatography , analytical chemistry (journal) , materials science , fetus , optics , biology , membrane , physics , pregnancy , genetics , biochemistry , optoelectronics
The present study was carried out for two reasons: to investigate the correlation between amniotic fluid lecithin/sphingomyelin (L/S) ratio and optical density at wavelengths ranging from 350 nm to 650 nm, and to determine the effect of centrifugal force on that correlation. When an L/S ratio of 2.0 and optical density values of 0.15 at 650 nm, 0.16 at 600 nm and 0.17 at 575 nm were taken as discriminative values between pulmonary maturity and immaturity, the true positive rate of samples centrifuged at 450xg was not different from that of samples centrifuged at 2000xg, but the false negative rate was high among the samples centrifuged at 2000xg (P>0.005). These results indicate that for amniotic fluid centrifuged at 450xg, the optical density at wavelengths ranging from 575 nm to 650 nm provides a rapid, simple, accurate indicator of fetal lung maturity.