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Long‐term Culture of Human Ovarian Theca Cell Tumor, an Estrogen‐producing Tumor
Author(s) -
Ishiwata Isamu,
Ishiwata Chieko,
Kobayashi Noboru,
Ishikawa Hiroshi
Publication year - 1983
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1983.tb00660.x
Subject(s) - estrogen , hyperplasia , ovary , theca , endocrinology , endometrial hyperplasia , cell , biology , in vitro , medicine , cell culture , andrology , chemistry , biochemistry , genetics
The cultured cells designated HKOT were derived from the ovarian theca cell tumor of a 78‐year‐old female. HKOT cells grew slowly for 6 weeks, and were maintained in vitro for about 16 weeks. The monolayer cultured cells were fibroblastic in shape and contained lipid droplets detected by Sudan red stain. The cells were diploid and did not show karyological abnormalities. The cells continued secreting primarily estrogens, and only small quantities of androgens, if any at all for about 40 days. The ectocervical hyperkeratosis and endometrial hyperplasia were considered to be attributable to the effects of estrogens.