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Immunoperoxidase Localization of Prolactin in Syncytio‐trophoblast Cells of Normal Pregnancy, Aborted Pregnancy, Hydatidiform Mole and Choriocarcinoma
Author(s) -
Tomoda Shoji,
Hamada Kazutaka,
Sugawa Tadashi,
Takahashi Kenichi P.,
Yamagata Kenzo
Publication year - 1983
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1983.tb00611.x
Subject(s) - syncytiotrophoblast , trophoblast , choriocarcinoma , immunoperoxidase , decidua , prolactin , pregnancy , molar pregnancy , chorioepithelioma , andrology , fetus , chemistry , biology , medicine , endocrinology , placenta , antibody , immunology , gestation , hormone , monoclonal antibody , genetics
In this study the histochemical immunoperoxidase method was adopted to clarify the localization of prolactin (PRL) in the decidua and villus of normal, aborted, and molar pregnancy and choriocarcinoma. After evacuation and curettage or operative resection, tissue specimens were fixed in formalin solution and embedded in paraffin. Some frozen sections were made after fixation by Zamboni's solution. The indirect immunoperoxidase method was used. Anti‐PRL antibody was donated by NIAMDD and was used after absorption by hPL. Results showed that: (1) In normal pregnancy, PRL is located in syncytiotrophoblast cells. (2) In spontaneous abortion, PRL is detected in syncytiotrophoblast cells, despite fetal death, provided that hCG continues to be produced. (3) In molar pregnancy, PRL is localized in syncytiotrophoblast cells, even those which penetrate into the decidua. (4) In choriocarcinoma, PRL is located in the cancer cells. We concluded that syncytiotrophoblast cells are the main site of PRL as long as trophoblast cells are alive and active.