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Synthesis of a tetratriacontapeptide corresponding to sequence 112–145 of the β ‐subunit of hCG and production of antiserum against this synthetic peptide
Author(s) -
Yamaji Kenji,
Takagi Tetsu,
Sugita Nagatoshi,
Tanizawa Osamu,
Okada Yoshio,
Kawasaki Koichi,
Iguchi Shin,
Yagyu Masami
Publication year - 1982
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1982.tb00579.x
Subject(s) - immunogen , antiserum , radioimmunoassay , carbodiimide , glutaraldehyde , bovine serum albumin , chemistry , protein subunit , microbiology and biotechnology , biology , endocrinology , medicine , biochemistry , chromatography , immunology , antibody , monoclonal antibody , gene
A tetratriacontapeptide(P‐34) corresponding to sequence 112–145 of the β ‐subunit of hCG(hCG‐ β ) was synthesized by the fragment condensation method. The immunogen was prepared by coupling P‐34 to bovine serum albumin by the carbodiimide or glutaraldehyde method. Antiserum was produced by injecting the immunogen into rabbits at multiple intradermal and intrasplenal sites with Freund's complete adjuvant. Eight weeks after the first immunization, antiserum was obtained that bound 30% of 125 I‐hCG or 125 I‐hCG‐ β at a dilution of 1:1,000. This binding was inhibited dose‐dependently by hCG. In this radioimmunoassay system, the minimal detectable dose of hCG was 500 mIU/ml and, as expected theoretically, no binding to 125 I‐hCG‐α or 125 I‐hLH was observed.