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Quantitative High‐Performance Liquid Chromatographic Determination of Δ 4 ‐3 Ketonic Free Steroids in Human Plasma and Amniotic Fluid
Author(s) -
Sagara Yusuke,
Yamanaka Satoshi,
Takeda Yoshihiko,
Tsuruta Hisao
Publication year - 1982
Publication title -
asia‐oceania journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 0389-2328
DOI - 10.1111/j.1447-0756.1982.tb00553.x
Subject(s) - chromatography , chemistry , amniotic fluid , methanol , absorption (acoustics) , steroid , cortisone , high performance liquid chromatography , corticosterone , elution , extraction (chemistry) , endocrinology , biochemistry , materials science , fetus , organic chemistry , hormone , biology , pregnancy , genetics , composite material
Summary A high‐performance liquid chromatographic method is described for the measurement and identification of eight steroids possessing the Δ 4 ‐3 ketonic compounds in human specimens. The eight steroids (Cortisone, Cortisol, Corticosterone, DOC, Δ 4 ‐Androstendione, Testosterone, 17‐Hydroxyprogesterone and Progesterone) were separated with a methanol‐THF‐water system on an IRICA RP‐18 column. Effluents were detected by UV absorption at 245 nm. Quantitative analysis was performed by measurement of peak heights, which were proportional to the amount of each substance. This method is rapid, highly sensitive, easy to perform and reproducible.