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Telomerase activity and telomere length distribution in vascular endothelial cells in a short‐term culture under the presence of hydrogen peroxide
Author(s) -
Maeda Toyoki,
Guan JingZhi,
Koyanagi Masamichi,
Makino Naoki
Publication year - 2013
Publication title -
geriatrics and gerontology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 57
eISSN - 1447-0594
pISSN - 1444-1586
DOI - 10.1111/j.1447-0594.2012.00936.x
Subject(s) - telomerase , telomere , hydrogen peroxide , oxidative stress , endothelial stem cell , microbiology and biotechnology , cell culture , cell , umbilical vein , chemistry , biology , biochemistry , dna , genetics , gene , in vitro
Aim: The aim of this study was to assess the biological effects of oxidative stress on human vascular endothelial cells. Methods: The telomeric changes and the alterations of the expression of telomere‐associated proteins in human umbilical venous endothelial cells (HUVEC) cultured in the presence of hydrogen peroxide (H 2 O 2 ) were analyzed. Results: During the culture, the cell growth rate decreased, whereas the telomerase activity of the surviving cells increased. As the H 2 O 2 level increased, long telomeres decreased proportionally, thus resulting in a telomere length distribution that was rich in short telomeres. These observations suggested that H 2 O 2 ‐affected endothelial cells bear telomeric features similar to those of aged cells. In contrast, the expression of telomere‐associated proteins, TRF1 and TRF2, showed different changes. TRF1 increased in relation to H 2 O 2 concentration, whereas TRF2 showed no significant change. The surviving cells exposed to H 2 O 2 showed a H 2 O 2 ‐dose dependent increase in telomerase activity, whereas the telomere protein and RNA components were only elevated in low concentrations of H 2 O 2 . Conclusions: The increase in telomerase activity and TRF1 protein expression of vascular endothelial cell might show an aspect of cellular protective reaction against oxygen stress. Geriatr Gerontol Int 2013; 13: 774–782.