Open AccessEffect of total cholesterol, glucose and blood urea nitrogen on embryo quality in post‐partum superovulated suckling Japanese Black cattle
Author(s) -
PRADHAN RAJANI,
OSHIMA KAZUNAGA,
OCHIAI YOSHINORI,
KOJIMA TAKATOSHI,
YAMAMOTO NAOYUKI,
GHANEM MOHAMED ELSHABRAWY,
NAKAGOSHI NOBUKAZU
Publication year - 2008
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1111/j.1447-0578.2008.00201.x
Subject(s) - ice calving , estrous cycle , corpus luteum , insemination , endocrinology , medicine , embryo quality , artificial insemination , estradiol benzoate , embryo , hormone , zoology , biology , pregnancy , andrology , embryogenesis , lactation , genetics , ovariectomized rat , microbiology and biotechnology
Aim: This study was conducted to examine the effect of blood metabolites on embryo quality in post‐partum suckling Japanese Black cattle. Methods: Blood samples were taken from 23 cows 30 days before, at and 30 days after parturition. Cows were synchronized 40 or 41 days after calving (day 0) and divided into three groups: control ( n = 6), gonadotropin‐releasing hormone ([GnRH] n = 10) and estradiol benzoate ([EB] n = 7). All groups received a controlled internal drug release (CIDR) device intravaginally together with 2 mg EB i.m. on day 0 and superovulation was induced in all groups from days 5–7 with a gradually decreasing dose of follicle‐stimulating hormone (FSH). Two milligrams of EB was given on day 8 and GnRH (0.1 mg) was given on day 9 of insertion of the CIDR in the EB and GnRH groups. Cows were inseminated twice after the onset of estrus and embryos were recovered 7–8 days after artificial insemination. Results: The number of corpus luteum detected by ultrasonography in the EB group was significantly higher ( P < 0.05) than that in the GnRH group. The number and rate of transferable and freezable embryos did not differ significantly among the groups. Regardless of the treatments, the total cholesterol level from parturition until 30 days after parturition was significantly higher ( P < 0.01) in the good category than in the poor category of cows. Conclusions: The number of transferable embryos produced by post‐partum superovulated suckling Japanese Black cattle was affected by the level of total cholesterol from parturition until 30 days after parturition. Moreover, administration of EB in CIDR‐treated cows increased the numbers of corpus luteum and yielded better rates of transferable and freezable embryos. (Reprod Med Biol 2008; 7 : 55–62)