Open AccessIn vitro growth and maturation of mouse oocyte‐granulosa cell complex from cryopreserved ovaries and achievement of pup birth
Author(s) -
HASEGAWA AKIKO,
KOYAMA KOJI
Publication year - 2007
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1111/j.1447-0578.2007.00169.x
Subject(s) - fertility preservation , premature ovarian failure , cryopreservation , oocyte , ovarian tissue cryopreservation , oocyte cryopreservation , in vitro fertilisation , ovary , andrology , in vitro maturation , live birth , ovarian cancer , reproductive medicine , assisted reproductive technology , ovarian tissue , medicine , gynecology , biology , embryo , cancer , fertility , infertility , pregnancy , microbiology and biotechnology , genetics , population , environmental health
Ovarian tissue banking is a feasible strategy for fertility preservation for young women after cancer treatments. Ovarian tissue, after thawing, is used for several options; orthotopic grafting (normal site), autologous heterotopic grafting and collection of ovarian follicles for culture. Recent reports of live birth encouraged clinicians and researchers to apply this technology to premature ovarian failure (POF) resulting from strong cancer therapy. Grafting, however, carries a risk of malignant cell recurrence. For safety, development of a culture method is necessary but optimum culturing conditions for less‐developed follicles abundant in the ovary are not well known. In the present article, the current status of ovarian tissue cryopreservation, and in vitro oocyte growth and maturation from the preserved ovaries are reviewed.