Open AccessEffects of dibromoacetic acid on murine spermatozoa and testis
Author(s) -
FUJINOKI MASAKATSU,
IMAIZUMI YOSHIE,
OHTAKE HIDEKI,
YAMAOKA SADAO
Publication year - 2004
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1111/j.1447-0578.2004.00059.x
Subject(s) - reproductive medicine , chemistry , microbiology and biotechnology , andrology , biology , medicine , genetics , pregnancy
Background and Aims: Bromoacetic acids are a by‐product of water ozonation and dibromoacetic acid (DBAA) in particular, which is a by‐product of disinfection, inhibits male reproductive functions. In order to understand its effects, the spermatozoa and testes of mice were exposed to DBAA. Methods: Twelve‐week‐old ICR mice were exposed to 10 p.p.m. DBAA. They were examined in regards to effects on the weights of body, testis and epididymis, the histological changes of tesits and the protein expression in testis. Results: Neither the bodyweight nor the weights of the testis and epididymis of the exposed mice was affected, but approximately 13% of spermatozoa obtained from the cauda epididymis were motile with a drop‐shaped head, and structures resembling residual bodies were found in the testis. Moreover, the expression of two testis proteins was changed by exposure to DBAA. Conclusions: It was likely that DBAA inhibited male reproductive functions by disturbance of spermatogenesis via change of protein expression. (Reprod Med Biol 2004; 3: 85–93)