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Influence of angiotensin converting enzyme (ACE) genotype on interpretation of diagnostic tests for serum ACE activity
Author(s) -
Stokes G. S.,
Monaghan J. C.,
Schrader A. P.,
Glenn C. L.,
Ryan M.,
Morris B. J.
Publication year - 1999
Publication title -
australian and new zealand journal of medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 70
eISSN - 1445-5994
pISSN - 0004-8291
DOI - 10.1111/j.1445-5994.1999.tb00713.x
Subject(s) - genotype , medicine , angiotensin converting enzyme , genotyping , peptidyl dipeptidase a , ace inhibitor , reference range , endocrinology , biology , genetics , gene , blood pressure
Background : The discovery that an insertion/deletion ( I/D ) polymorphism of the angiotensin converting enzyme (ACE) gene influences the circulating concentration of ACE may have implications for the proper use of serum ACE activity measurements in screening for sarcoidosis. Aim : To determine whether the sensitivity of the serum ACE test improves if ACE genotype is taken into account. Methods : A retrospective determination of ACE genotype and clinical diagnosis was done in 54 patients with serum ACE activity above the upper limit of the reference range for the insertion ( II ) genotype. ACE was measured by radioenzymatic and spectrophotometric techniques, and genotype by PCR. Results : When serum ACE values determined diagnostically were related to the appropriate genotype‐specific reference range, sensitivity and specificity for diagnosis of sarcoidosis were 65–70% and 58% respectively, compared to 47–57% and 77% with a reference range unsegregated for genotype. Conclusion : ACE genotyping may be helpful in determining the diagnostic significance of mildly elevated serum ACE activity in patients with the II and ID genotypes.