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Pregnancy Associated Plasma Protein‐A: Interaction with Heparin in Crossed Affinity lmmunoelectrophoresis
Author(s) -
Sinosich M. J.,
Teisner B.,
Davey M.,
Grudzinskas J. G.
Publication year - 1981
Publication title -
australian and new zealand journal of medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 70
eISSN - 1445-5994
pISSN - 0004-8291
DOI - 10.1111/j.1445-5994.1981.tb03527.x
Subject(s) - heparin , antithrombin , pregnancy associated plasma protein a , glycoprotein , alpha (finance) , pregnancy , blood proteins , immunoelectrophoresis , placenta , medicine , fetus , endocrinology , antibody , immunology , biology , biochemistry , surgery , first trimester , genetics , construct validity , patient satisfaction
A specific interaction between pregnancy associated plasma protein‐A (PAPP‐A) arid heparin has been demonstrated using heparin affinity crossed immuno electrophoresis applied to late pregnancy serum. The presence of heparin in the first dimensional gel accelerated the anodic migration of six serum proteins with β electrophoretic mobility and one protein in the α mobile region. Two of the six β mobile proteins were identified as anti‐thrombin III and β lipoprotein, the α mobile protein being PAPP‐A. The migration distance of other proteins originating in the placenta (pregnancy specific β 1 glycoprotein), fetus (alpha‐fetoprotein). leucocytes (pregnancy zone protein), or maternal liver (α 1 antitrypsin α 2 macroglobulin) was not altered. The interaction of PAPP‐A with heparin was therefore independent of Molecular size, charge, and site of origin indicating a specific high affinity interaction between PAPP‐A and heparin. These results indicate that PAPP‐A may be involved locally with the coagulation system in the maintenance of placental circulation .