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*THE PROTEOME OF MESENTERIC LYMPH DURING ACUTE PANCREATITIS AND IMPLICATIONS FOR TREATMENT
Author(s) -
Mittal A.,
Phillips A.,
Middleditch M.,
Rugeiro K.,
Loveday B.,
Windsor J. A.
Publication year - 2009
Publication title -
anz journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.426
H-Index - 70
eISSN - 1445-2197
pISSN - 1445-1433
DOI - 10.1111/j.1445-2197.2009.04920_5.x
Subject(s) - acute pancreatitis , medicine , lymph , pancreatitis , proteome , gastroenterology , mesenteric lymph nodes , pathology , bioinformatics , biology , spleen
Background: Mesenteric lymph has been implicated in the development of organ dysfunction in acute pancreatitis and critical illness. The protein fraction of mesenteric lymph is thought to contain toxic factors. However, we do not have a complete description of the mesenteric lymph proteome during acute pancreatitis. Aim: The aim of this study was to identify significant changes in the mesenteric lymph proteome during acute pancreatitis. Methods: Mesenteric lymph was collected from sixteen male Wistar rats randomised to Group 1 (n = 8) sham control and Group 2 (n = 8) with taurocholate induced acute pancreatitis. The lymph was subjected to proteomic analysis using iTRAQ(tm) and liquid chromatography–tandem mass spectrometry. Results: 245 proteins including 35 hypothetical proteins were identified in mesenteric lymph. There was a significant increase in the relative abundance of 8 of the 245 proteins in acute pancreatitis conditioned mesenteric lymph, and 7 of these were pancreatic catabolic enzymes (pancreatic amylase 2, pancreatic lipase, carboxypeptidase A2, chymotrypsinogen B, carboxypeptidase B1, cationic trypsinogen, ribonuclease 1). Conclusions: This is the first comprehensive description of the proteome of mesenteric lymph during acute pancreatitis. The striking presence of pancreatic catabolic enzymes in the mesenteric lymph, and the absence of other candidate proteins, provide incentive to explore the role of these proteases in the development of multiple organ dysfunction and provide a rationale for further studies to explore a potential therapeutic role for enteral anti‐protease therapy in acute pancreatitis.