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EFFECT OF N ‐ACETYL‐ l ‐CYSTEINE ON LUNG ISCHAEMIA REPERFUSION INJURY IN A PORCINE EXPERIMENTAL MODEL
Author(s) -
Chamogeorgakis Themistocles P.,
Kostopanagiotou Georgia G.,
Kalimeris Constantine A.,
Kabouroglou George I.,
Kourtesis Antonios N.,
Routsi Christine I.,
Dima Cleanthi C.,
Toumpoulis Ioannis K.
Publication year - 2008
Publication title -
anz journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.426
H-Index - 70
eISSN - 1445-2197
pISSN - 1445-1433
DOI - 10.1111/j.1445-2197.2007.04357.x
Subject(s) - medicine , cysteine , reperfusion injury , ischemia , myocardial reperfusion injury , lung , anesthesia , pharmacology , cardiology , biochemistry , enzyme , chemistry
Background: The purpose of the present study was to investigate the effect of N ‐acetyl‐ l ‐cysteine on lung ischaemia reperfusion injury. Methods: Nineteen pigs were used. Group I ( n = 5) underwent sham operation, group II ( n = 7) 90‐min left‐lung ischaemia followed by 180‐min reperfusion. In group III ( n = 7) N ‐acetyl‐ l ‐cysteine was given (160 mg/kg) during ischaemia into the pulmonary artery. Lung‐functional and haemodynamic parameters were measured; serum and lung tissue samples were obtained and analysed for interleukin‐10 and tumour necrosis factor‐α. At the end of the reperfusion bronchoalveolar lavage was carried out from the ipsilateral lung and analysis for total protein, phospholipase‐A 2 and platelet‐activating factor acetylhydrolase was carried out. Histological specimens were graded (0–3) for alveolar oedema, interstitial thickening and leucocyte infiltration. Statistical analysis was by means of one‐way analysis of variance and Kruskal–Wallis test. Results: There were no differences in haemodynamic parameters, serum and tissue interleukin‐10 and tumour necrosis factor‐α. Pulmonary compliance was decreased in groups II and III ( P = 0.002 and P = 0.001, respectively) during ischaemia and reperfusion. Pulmonary vascular resistance was increased in group II ( P = 0.051) during reperfusion. In group III total protein and platelet‐activating factor acetylhydrolase were increased ( P = 0.004 and P = 0.006, respectively) and phospholipase‐A 2 was reduced ( P = 0.002), indicating an indirect surfactant‐protective effect. Interstitial thickening was excessive in group II ( P = 0.001); however, alveolar oedema was reduced ( P = 0.002) when compared with group III. Conclusion: N ‐acetyl‐ l ‐cysteine when administered directly in the pulmonary artery showed no significant change in haemodynamic and functional lung parameters during ischaemia reperfusion; it does, however, have an indirect surfactant‐protective effect.