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PRECLINICAL TRIAL OF A BlOARTlFlClAL LIVER SUPPORT SYSTEM IN A PORCINE FULMINANT HEPATIC FAILURE MODEL
Author(s) -
Sheil A. G. R.,
Sun J.,
Mears D. C.,
Warin M.,
Woodman K.,
Johnston B.,
Horvat M.,
Watson K. J.,
Koutalistras N.,
Wang LS.
Publication year - 1996
Publication title -
australian and new zealand journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.111
H-Index - 51
eISSN - 1445-2197
pISSN - 0004-8682
DOI - 10.1111/j.1445-2197.1996.tb00807.x
Subject(s) - fulminant hepatic failure , bioartificial liver device , medicine , extracorporeal , collagenase , toxicity , bioreactor , andrology , hepatocyte , biochemistry , in vitro , biology , liver transplantation , transplantation , enzyme , botany
Background: This study describes the pre‐clinical trials of an extracorporeal bioartificial liver support system (BALSS). It includes the biochemical changes which occur in the plasma and blood of pigs with devascularized livers when the plasma is treated in a BALSS, and the testing of the system for presence or absence of infective agents, pyrogens and for toxicity. Methods: Hepatic cells were prepared from littermate juvenile white landrace pigs with a double‐step collagenase digest technique. The cell preparations were incubated with collagen‐coated dextran microspheres (CDM) for 3 h and the medium was tested to determine cellular metabolic activity. Incubation continued for a further 20 h during which the hepatic cells attach to the CDM. The CDM‐attached cells were inoculated into a hollow fibre bioreactor which was part of an extracorporeal liver support system. Results: Hepatic cell content of the bioreactor was 6 × 10 9 ± 3 × 10 18 cells, equivalent to those present in half a pig's liver. The system was tested in a controlled trial with the plasma of pigs with fulminant hepatic failure (FHF) due to devascularized livers. When plasma from FHF pigs was circulated through the device there was significantly less of an increase in the accumulation of ammonia, lactate and most amino acids when hepatic cells were included in the circuit compared with those in control experiments when they were excluded. Similar changes occurred in porcine blood. There were few infections diagnosed and an absence of pyrogens, endotoxins and toxicity in the bioreactor contents or in the terminating reservoir or animal blood samples. Conclusions: We believe that the results, demonstrating function of the porcine hepatic cells in the circuit, together with low risks, justify a clinical trial of use of the BALSS in Australia.