Premium
A COMPARISON OF FLUSHING SOLUTIONS FOR LIVER PROCUREMENT USING AN ISOLATED PERFUSED PORCINE MODEL
Author(s) -
Bell Roger,
David Nerissa,
Burrows Peter,
House Anthony K.
Publication year - 1994
Publication title -
australian and new zealand journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.111
H-Index - 51
eISSN - 1445-2197
pISSN - 0004-8682
DOI - 10.1111/j.1445-2197.1994.tb02288.x
Subject(s) - viaspan , flushing , perfusion , medicine , potassium , andrology , zoology , urology , chromatography , endocrinology , transplantation , chemistry , biology , organic chemistry
There is no agreement on the best technique of in situ flushing of livers prior to storage. In order to study this, porcine livers were stored in the University of Wisconsin (UW) solution for 18h and then assessed using an isolated perfused porcine model. Livers flushed in situ with UW solution were compared to livers flushed with a non‐preservation solution (Hartmann's solution). No statistically significant differences could be found in bile production (18.7 ± 4.4 vs 17.9 ± 3.8 mL/1000g per 2h), aspartate amino‐transferase (AST) levels in the perfusate after 2h of isolated perfusion (687 ± 101 vs 724 ± 114U/L), potassium levels in the perfusate after 2h on the circuit (5.4 ± 1.5 vs 5.5 ± 2.3mmol/L), weight gain (15.2 ± 3.7 vs 17.1 ± 4.0%) or platelet sequestration (41.6 ± 11.7 vs 37.4 ± 9.8%) between livers flushed with UW solution as opposed to those flushed with Hartmann's solution, respectively. Of overriding importance was the solution in which the liver was stored, reconfirming the superiority of UW solution over an extracellular solution for preservation. If extrapolated to the clinical situation, these findings would have substantial cost‐saving implications.