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ELASTASE LEVELS IN SALVAGED BLOOD AND THE EFFECT OF CELL WASHING
Author(s) -
Sieunarine K.,
Langton S.,
LawrenceBrown M. M. D.,
Goodman M. A.,
Prendergast F. J.,
Hellings M.
Publication year - 1990
Publication title -
australian and new zealand journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.111
H-Index - 51
eISSN - 1445-2197
pISSN - 0004-8682
DOI - 10.1111/j.1445-2197.1990.tb07441.x
Subject(s) - medicine , elastase , blood cell , white blood cell , cell , autologous blood , surgery , enzyme , immunology , biochemistry , chemistry
Blood was salvaged from the operating field of 16 orthopaedic and vascular operations and processed by the Shiley Dideco cell saver for retransfusion. Plasma elastase levels in the salvaged blood were used as a marker of white cell lysosomal granule release. The plasma level of this enzyme was measured by the technique described by Dreher et al . using the immuno‐activation (IMAC™) system (Merck‐Darmstadt, Federal Republic of Germany). 1 The levels of the enzyme rose dramatically in the salvaged blood and were reduced to preoperative levels after washing in the cell saver. Washing of the salvaged blood before retransfusion is effective in reducing the plasma levels of white cell lysosomal contents which have the potential for producing harmful systemic effects.