Premium
REVIEW OF KERATINOCYTE CULTURE TECHNIQUES: PROBLEMS OF GROWING SKIN
Author(s) -
Breidahl A. F.,
Juoson R. T.,
Clunie G. J. A.
Publication year - 1989
Publication title -
australian and new zealand journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.111
H-Index - 51
eISSN - 1445-2197
pISSN - 0004-8682
DOI - 10.1111/j.1445-2197.1989.tb01615.x
Subject(s) - medicine , keratinocyte , cell culture , biology , genetics
Keratinc cyte cell culture has been studied by a large number of investigators, resulting in an extensive body of often contradictory literature. The advantages and disadvantages of the two major techniques, explain and disaggregated culture, will be examined, followed by a critical evaluation of the major technical variables. Suggested standardized methods for both explant and disaggregated culture, and the indications for the use of each, will be proposed. Finally, further directions for basic research will be suggested in order to improve this useful tcol.