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A TECHNIQUE FOR THE IN VITRO CULTURE OF HUMAN PARATHYROID GLAND TISSUE
Author(s) -
Matalanis George,
Clunie Gordon J. A.,
Whitehead Robert H.
Publication year - 1988
Publication title -
australian and new zealand journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.111
H-Index - 51
eISSN - 1445-2197
pISSN - 0004-8682
DOI - 10.1111/j.1445-2197.1988.tb01089.x
Subject(s) - parathyroid chief cell , hypoparathyroidism , in vitro , parathyroid hormone , medicine , tissue culture , parathyroid gland , transplantation , endocrinology , calcium , biology , biochemistry
A technique for prolonged in vitro culture of human parathyroid tissue is described. Cells could be maintained in this monolayer system and were shown to continue releasing high levels of parathyroid hormone into their culture medium even after 140 days in culture. Furthermore, growth of fibroblasts, persistence of epithelial cells and parathyroid hormone release was demonstrated in cells derived from parathyroid tissue that had been cryopreserved for 2 years. The availability of viable and functioning human parathyroid tissue produced in this culture system may be of value in both auto‐ and allo‐transplantation in patients with permanent hypoparathyroidism.