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G CELLS AND GASTRIN RELEASE FROM HUMAN ANTRAL MUCOSA IN VITRO
Author(s) -
Askew A. R.,
Grant B. J.,
Vinik A. I.
Publication year - 1980
Publication title -
australian and new zealand journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.111
H-Index - 51
eISSN - 1445-2197
pISSN - 0004-8682
DOI - 10.1111/j.1445-2197.1980.tb04128.x
Subject(s) - gastrin , stimulation , in vitro , antrum , perfusion , medicine , endocrinology , population , cell , g cell , choline , andrology , biology , biochemistry , stomach , secretion , environmental health
Using an in vitro perfusion technique, the release of immunoreactive gastrin (IRG) from human antral mucosa has been investigated, and comparisons made between the release of IRG and the G cell numbers within the tissue. Serosal stimulation with acetyle choline(5·504 × 10 ‐12 M conc.)resulted in a significantly greater release of IRG from the tissue when compared with release from unstimulated tissue from the same patient (4666 ± 1656 fM/ml. stimulated; 274 ± 130 fM/ml. unstimulated (T = 2·64; P (0·02). A wide variation in G cell numbers was found between tissues and within tissue, and there was no significant correlation between the gastrin secreted and the G cell population. Stimulation with acetyl choline resulted in a 68·8% reduction in G cell numbers compared with a 56·7% reduction in unstimulated tissue, but these were not significantly different (T = 1·68; P) 0·1). No significant correlation was found between the reduction in G cell numbers detected by immunohistology and the IRG secreted by the tissues.