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TRANSPLANTATION OF ORGAN CULTURED RAT PARATHYROIDS
Author(s) -
GOUGH IAN R.,
FlNNIMORE MARGARET
Publication year - 1979
Publication title -
australian and new zealand journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.111
H-Index - 51
eISSN - 1445-2197
pISSN - 0004-8682
DOI - 10.1111/j.1445-2197.1979.tb06501.x
Subject(s) - immunogenicity , medicine , organ culture , in vitro , transplantation , rat model , tissue culture , andrology , immunology , antigen , biology , biochemistry
The hypothesis that tissue culture alters the immunogenicity of grafts by removal or inactiva‐tion of passenger leucocytes has been investigated in an inbred rat parathyroid allograft model. DA rat (Ag‐B 4 ) parathyroid glands cultured for up to three weeks in Eagle's minimal essential medium (MEM) alone, or MEM with added donor‐specific antilymphocyte serum, did not survive longer than fresh glands when allografted into Lewis rat (Ag‐B 1 ) recipients. Subsequent in vitro comparisons of three different culture media established that RPM11640 was superior to MEM and Medium 199 for rat parathyroids. However, further series of transplants after culture in RPM11640 alone also failed to produce prolongation of allograft survival.