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C‐reactive protein may be a marker of bacterial translocation in experimental intestinal obstruction
Author(s) -
Çevikel Mehmet Hakan,
Özgün Hedef,
Boylu Sükrü,
Demirkiran Ahmet Ender,
Aydin Neriman,
Sari Cavide,
Erkus Muhan
Publication year - 2004
Publication title -
anz journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.426
H-Index - 70
eISSN - 1445-2197
pISSN - 1445-1433
DOI - 10.1111/j.1445-1433.2003.02681.x
Subject(s) - medicine , bacterial translocation , mesenteric lymph nodes , gastroenterology , lamina propria , c reactive protein , chromosomal translocation , pulmonary sequestration , laparotomy , bowel obstruction , surgery , pathology , inflammation , lung , spleen , epithelium , biology , biochemistry , gene
Background: C‐reactive protein (CRP) is used as a marker of intestinal ischaemia. This study evaluated whether CRP levels can be used to detect ischaemia‐induced (strangulated) intestinal obstruction and subsequent bacterial translocation. Methods: Forty‐eight rats, divided into four groups underwent the following procedures: anaesthesia alone (native controls), laparotomy (sham‐operated controls), or surgical induction of simple or strangulated intestinal obstruction (simple and strangulated obstruction groups, respectively). Blood samples were collected for culture and serum CRP analysis. In addition, liver and mesenteric lymph node (MLN) specimens were collected for culture, to determine the presence of bacterial translocation; and ileal segments, for histopathological investigation. Results: CRP levels and rates of bacterial translocation, expressed as colony forming units (cfu) per gram wet tissue, were higher in both intestinal obstruction groups than in the native and sham‐operated control groups ( P < 0.001 for both). The increases in CRP levels paralleled increases in the number of cfu in the MLN and liver cultures ( P < 0.01). Compared to controls, animals in the obstruction groups also had a higher incidence of positive blood cultures ( P < 0.005) and greater histopathologic evidence of inflammatory infiltration of the lamina propria ( P < 0.01). However, no significant difference between the simple and strangulated obstruction groups was observed. Conclusion: CRP levels increase with the severity of bacterial translocation in acute intestinal obstruction but do not permit discrimination between simple and strangulated intestinal obstruction.