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Immunohistochemical Identification of S Phase Cells in the Gastric Mucosa Using Bromodeoxyuridine and Its Monoclonal Antibody
Author(s) -
WANG JinTown,
SHEU JinChuan,
SHUN ChiaTung,
LIN JawTown,
WANG ChengYi,
WANG TehHong
Publication year - 1991
Publication title -
digestive endoscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.5
H-Index - 56
eISSN - 1443-1661
pISSN - 0915-5635
DOI - 10.1111/j.1443-1661.1991.tb00284.x
Subject(s) - bromodeoxyuridine , antrum , immunohistochemistry , gastric mucosa , pathology , monoclonal antibody , fundus (uterus) , medicine , stomach , staining , biopsy , antibody , immunology , ophthalmology
Using in vitro bromodeoxyuridine (BrdU) labelling of endoscopic biopsied gastric mucosal tissue and immunohistochemical staining using a monoclonal antibody against BrdU, we studied the S phase fraction of normal gastric mucosa. Twenty‐one patients who had undergone an endoscopic examination and who had normal gross mucosa were biopsied from the antrum, body and fundus. Eighteen of the 21 subjects were successfully labelled using BrdU in a tissue culture medium, sectioned in a longitudinal orientation and stained using the peroxidase‐anti‐peroxidase complex method using a monoclonal antibody against BrdU. The mean labelling index of these. 18 subjects was 36.1 ± 15.3, 21.2 ± 8.2, 23.0 ± 11.5 per 1000 nuclei for the mucosa of the antrum, body and fundus, respectively. The S phase fraction of the antral mucosa was significantly higher than the mucosa of the body and fundus. We conclude that the BrdU‐anti‐BrdU monoclonal antibody method is stable and rapid in the study of cell proliferation of gastric mucosa and the S phase fraction in antral mucosa is higher than those of the body and fundus. The biological significance of this finding needs further investigation.