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A Survey of Regenerating Capacity in Patchy Liver and in Nodular Liver—with Special Reference to DNA Synthesis Estimated by BrdU Labeling Index—
Author(s) -
TARAO Kazuo,
SHIMIZU Akio,
KUNI Yukifusa,
ITO Yoshihiko,
TAMAI Setsuo,
IIMORI Kazuto,
KUBO Kiyoshi,
TAKAGI Seiichi,
OIKAWA Yuko,
KIYOHASHI Atsushi,
UNAYAMA Shiro,
HOSHINO Hiroshi,
MIZUNO Tadaoki,
SHINDO Kunihiko
Publication year - 1989
Publication title -
digestive endoscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.5
H-Index - 56
eISSN - 1443-1661
pISSN - 0915-5635
DOI - 10.1111/j.1443-1661.1989.tb00036.x
Subject(s) - liver biopsy , pathology , immunohistochemistry , liver regeneration , biopsy , bromodeoxyuridine , lobules of liver , medicine , biology , regeneration (biology) , microbiology and biotechnology
: Laparoscopically, patchy liver and nodular liver were considered as features of regeneration of liver cells. In this study, the regenerating capacity of liver cells obtained by a liver biopsy from laparoscopically identified patchy liver and nodular liver was estimated by Bromodeoxvuridine (BrdU)‐anti‐BrdU method. BrdU labeling indices (L. I.), of liver cells in biopsy specimens from 6 normal livers, 12 patchy livers, and from 15 nodular livers were examined using an in‐vitro labeling technique. Liver biopsy specimens obtained by a Tru‐cut needle were immediately incubated for 45 min. in 0.1% BrdU solution in RPMI 1640 at 37°C under a pressure of 3 atmospheres in a mixture of 95% O 2 and 5% CO 2 . Immunohistochemical detection of BrdU was performed by the Avidin‐Biotin‐Peroxidase Complex (ABC) method. The mean BrdU L. I. (±SE) of normal liver, patchy liver, and of nodular liver was 0.25±0.09%, 1.4±0.2%, and 1.7±0.4% respectively. Among the nodular liver, flat shaped ones showed a low level (0.5±0.1%), in contrast to the high level (2.4±0.7%) in the semispherical nodular liver. The BrdU L. I. of both the patchy liver and the nodular liver was significantly higher than that in the normal liver (p<0.001, p<0.01 respectively). Interesting enough, there was a significant difference (p<0.05) between flat shaped nodular livers and semispherical ones. The semispherical nodular liver demonstrated the highest capacity of DNA synthesis in all the groups examined.