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Expression of advanced glycation end products and related molecules in diabetic fibrovascular epiretinal membranes
Author(s) -
Abu ElAsrar Ahmed M,
Missotten Luc,
Geboes Karel
Publication year - 2010
Publication title -
clinical and experimental ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.3
H-Index - 74
eISSN - 1442-9071
pISSN - 1442-6404
DOI - 10.1111/j.1442-9071.2010.02194.x
Subject(s) - medicine , immunohistochemistry , stromal cell , blood vessel , cd34 , tumor necrosis factor alpha , transforming growth factor , integrin , diabetic retinopathy , glycation , neovascularization , membrane , endocrinology , angiogenesis , diabetes mellitus , receptor , microbiology and biotechnology , biology , biochemistry , stem cell
A bstract Purpose: To investigate associations between expressions of advanced glycation end products (AGEs), transforming growth factor‐β (TGF‐β), tumour necrosis factor‐α (TNF‐α) and integrins and correlations between their expression and level of vascularization and proliferative activity in diabetic fibrovascular epiretinal membranes. Methods: Membranes from eight patients with active proliferative diabetic retinopathy and nine patients with inactive proliferative diabetic retinopathy were studied by immunohistochemistry. Results: Blood vessels expressed AGEs, TGF‐β, TNF‐α and α v β 3 integrin in 5, 13, 8 and 8 membranes, respectively. Stromal cells expressed AGEs, TNF‐α and α v β 3 integrin in 15, 13 and 3 membranes, respectively. There was no immunoreactivity for α v β 5 , α 5 β 1 and α 2 β 1 integrins. There were significant correlations between number of blood vessels expressing CD34 and number of blood vessels expressing AGEs ( r s = 0.496; P = 0.043), TGF‐β ( r s = 0.777; P < 0.001) and TNF‐α ( r s = 0.699; P = 0.002). There were significant correlations between number of blood vessels expressing AGEs and number of blood vessels expressing TGF‐β ( r s = 0.532; P = 0.028) and TNF‐α ( r s = 0.626; P = 0.007). The correlation between number of blood vessels expressing TNF‐α and α v β 3 integrin was significant ( r s = 0.617; P = 0.008). Number of blood vessels expressing CD34 ( P = 0.001), TGF‐β ( P = 0.006) and TNF‐α ( P = 0.002) and stromal cells expressing AGEs ( P = 0.001) and TNF‐α ( P = 0.004) were significantly higher in active membranes than in inactive membranes. Conclusion: Interactions of AGEs, TGF‐β, TNF‐α and α v β 3 integrin might be involved in pathogenesis of proliferative diabetic retinopathy fibrovascular proliferation.