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Effects of latanoprost, timolol and GLC756, a novel dopamine D 2 agonist and D 1 antagonist on LTC 4 release after rat mast cell activation
Author(s) -
Laengle Ulrich W,
Markstein Rudolf,
Pralet Dominique,
Seewald Wolfgang,
Roman Danielle
Publication year - 2007
Publication title -
clinical and experimental ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.3
H-Index - 74
eISSN - 1442-9071
pISSN - 1442-6404
DOI - 10.1111/j.1442-9071.2007.01560.x
Subject(s) - timolol , latanoprost , mast cell , pharmacology , medicine , antagonist , agonist , degranulation , endocrinology , immunology , receptor , glaucoma , ophthalmology
A bstract Purpose: Mast cells participate in ocular allergic inflammation by releasing biologically active mediators. Leukotrienes are released from activated mast cells via an IgE‐dependent mechanism, and play a crucial role in ocular allergic inflammation. In this study, the effect of three topical antiglaucoma drugs, that is, latanoprost, timolol and GLC756, a novel dopamine D 2 agonist and D 1 antagonist, on leukotriene C 4 (LTC 4 ) release after rat mast cell activation was examined. Methods: A rat basophilic leukaemia RBL‐2H3 mast cell line was activated via IgE/anti‐IgE. Rat mast cells were incubated with latanoprost, timolol, or GLC756 at concentrations of 0.1, 1, 10 and 30 μM. LTC 4 concentration in supernatant was assessed 5 h post activation by EIA. Results: Compared with controls, timolol showed no relevant effect on LTC 4 release, 5 h after mast cell activation. Latanoprost and GLC756, in contrast, revealed an inhibitory effect on LTC 4 release, which was dose‐related and statistically significant at the concentrations of 10 and 30 μM. Conclusion: The results of this study suggest that timolol has no significant influence on LTC 4 release from activated mast cells. By contrast, latanoprost and GLC756 inhibited LTC 4 release, suggesting a possible anti‐inflammatory effect on ocular allergic inflammatory processes in topical glaucoma medication.