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Methylenetetrahydrofolate reductase 677 C‐T and homocysteine levels in Turkish patients with pseudoexfoliation
Author(s) -
Turaçli M Erol,
Tekeli Oya,
Özdemir Filiz,
Akar Nejat
Publication year - 2005
Publication title -
clinical and experimental ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.3
H-Index - 74
eISSN - 1442-9071
pISSN - 1442-6404
DOI - 10.1111/j.1442-9071.2005.01070.x
Subject(s) - methylenetetrahydrofolate reductase , medicine , homocysteine , gastroenterology , pseudoexfoliation , pseudoexfoliation syndrome , prospective cohort study , glaucoma , allele , ophthalmology , genetics , gene , biology
A bstract Purpose: The 677 C‐T polymorphism in the methylenetetrahydrofolate reductase ( MTHFR ) gene is associated with hyperhomocysteinaemia. This study aimed to investigate the frequency of MTHFR 677 C‐T polymorphism and homocysteine (Hcy) levels in Turkish patients with pseudoexfoliation (PEX). Methods: This prospective study consisted of 60 patients with PEX glaucoma, 16 with PEX syndrome and 34 controls. All patients and control subjects were of Turkish origin. All participants underwent a complete ophthalmic examination. Plasma Hcy levels were measured using fluorescence polarization immunoassay (Abbott Diagnostics). Hyperhomocysteinaemia was defined as a plasma Hcy level above 14 µmol/L. MTHFR 677 C‐T was genotyped by real‐time polymerase chain reaction (Roche Diagnostics). Results: There was no statistically significant difference in patient and control groups for the age and sex ( P > 0.05). The mean plasma Hcy levels were 15.76, 14.98 and 17.43 µmol/L in patients with PEX glaucoma, with PEX syndrome and controls, respectively. No significant differences between Hcy levels among the three groups ( P > 0.05) were found. MTHFR 677 polymorphism did not show significant differences between the total patients with PEX (PEX glaucoma + PEX syndrome) and control subjects (CC: 51.3% n = 39, CT: 40.8% n = 31, TT: 7.9% n = 6 for patients with PEX; CC: 52.9% n = 18, CT: 35.3:% n = 12, TT: 11.8% n = 4 for controls) ( P > 0.05). Frequency of T allele was 28.28% and 29.41% for the total patients with PEX and controls, respectively. Conclusion: In Turkish patients with PEX, the authors could not find any association between Hcy levels, MTHFR 677 C‐T polymorphism, and the disease.