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Transplantation of mature adipocyte‐derived dedifferentiated fat (DFAT) cells improves urethral sphincter contractility in a rat model
Author(s) -
Obinata Daisuke,
Matsumoto Taro,
Ikado Yuichiro,
Sakuma Takahiro,
Kano Koichiro,
Fukuda Noboru,
Yamaguchi Kenya,
Mugishima Hideo,
Takahashi Satoru
Publication year - 2011
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2011.02865.x
Subject(s) - transplantation , medicine , regeneration (biology) , contractility , saline , urethral sphincter , urethra , pathology , endocrinology , anatomy , biology , microbiology and biotechnology
Objectives: To examine the effects of mature adipocyte‐derived dedifferentiated fat (DFAT) cell transplantation on urethral tissue regeneration and sphincter function. Methods: Sixteen female Sprague–Dawley rats underwent vaginal distension (VD) for 3 h. Subsequently, green fluorescence protein (GFP)‐labeled DFAT cells (1 × 10 6 in 20 µL saline, DFAT group, n = 8) or saline (20 µL, control group, n = 8) were injected into paraurethral connective tissue. Two weeks following VD, leak point pressure (LPP) was measured and an immunohistochemical analysis of the urethra was performed to evaluate urethral sphincter regeneration. Results: The VD model was characterized by atrophy of the urethral sphincter and showed a decrease in LPP. DFAT cell transplantation resulted in a significant improvement of LPP (DFAT group: 37.3 ± 6.4 vs control group: 21.7 ± 5.7 mmHg, P < 0.01). Immunohistochemistry revealed that the striated muscle thickness and smooth muscle α−actin‐positive area were significantly ( P < 0.05) larger in the DFAT group than in the control group. DFAT cell transplantation enhanced macrophage accumulation followed by an increased number of cells in the proliferative state. Transplanted DFAT cells were observed in the damaged smooth muscle layer and showed positive staining for smooth muscle α−actin, suggesting conversion into the smooth muscle cell phenotype. Conclusions: DFAT cell transplantation promotes sphincter muscle regeneration and improves LPP in the rat VD model.